Genetic evidence that the degradation ofpara-cresol byGeobacter metallireducensis catalyzed by the periplasmicpara-cresol methylhydroxylase
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منابع مشابه
p-cresol methylhydroxylase from a denitrifying bacterium involved in anaerobic degradation of p-cresol.
A bacterium, strain PC-07, previously isolated as part of a coculture capable of growing on p-cresol under anaerobic conditions with nitrate as the acceptor was identified as an Achromobacter sp. The first enzyme of the pathway, p-cresol methylhydroxylase, which converts its substrate into p-hydroxybenzyl alcohol, was purified. The enzyme had an Mr of 130,000 and the spectrum of a flavocytochro...
متن کاملPathways for the degradation of m-cresol and p-cresol by Pseudomonas putida.
A comparison of the oxidation rates of various compounds by whole cells of Pseudomonas putida 3, 5 indicated that m-cresol is metabolized by oxidation to 3-hydroxybenzoate followed by hydroxylation to gentisate, the ring-fission substrate, when grown with 3, 5-xylenol. However, when m-cresol was the growth substrate, similar experiments suggested a different pathway involving a methyl-substitut...
متن کاملAnaerobic oxidation of p-cresol mediated by a partially purified methylhydroxylase from a denitrifying bacterium.
Anoxic cell extracts of a denitrifying bacterial isolate (PC-07) were shown to oxidize p-cresol to p-hydroxybenzoate. Oxidation of the substrate was independent of molecular oxygen and required nitrate as the natural terminal electron acceptor. Two enzyme activities were implicated in the pathway utilized by PC-07. A p-cresol methylhydroxylase mediated the oxidation of p-cresol to p-hydroxybenz...
متن کاملSubunit interactions change the heme active-site geometry in p-cresol methylhydroxylase.
The enzyme p-cresol methylhydroxylase [4-cresol: (acceptor) oxidoreductase (methyl-hydroxylating), EC 1.17.99.1] contains two subunits: a cytochrome c (electron transfer) subunit (cytochrome cpc) and a flavin (catalytic) subunit. When these subunits are separated by isoelectric focusing, a stable cytochrome subunit is obtained. Significant differences are observed between the one-dimensional NM...
متن کاملAnaerobic Degradation of m-Cresol by a Sulfate-Reducing Bacterium.
m-Cresol metabolism under sulfate-reducing conditions was studied with a pure culture of Desulfotomaculum sp. strain Groll. Previous studies with a sulfate-reducing consortium indicated that m-cresol was degraded via an initial para-carboxylation reaction. However, 4-hydroxy-2-methylbenzoic acid was not degraded by strain Groll, and no evidence for ring carboxylation of m-cresol was found. Stra...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2015
ISSN: 1574-6968
DOI: 10.1093/femsle/fnv145